|
KMID : 0357119970190010121
|
|
Korean Journal of Immunology
1997 Volume.19 No. 1 p.121 ~ p.128
|
|
|
Differential Regulation of Antioxidant Enzymes during Monocyte Differentiation
|
|
Lee Young-Sup
|
|
|
Abstract
|
|
|
Antioxidant enzymes levels were determined in monocytes during phorbol myristate acetate (PMA)-induced differentiation. PMA induced the differentiation of a human monocytic leukemia cell line THP-1 into macrophage-like cells as indicated by
activity of acid phosphatase and morphological changes. The level of Mn-superoxide dismutase (SOD) was selectively increased in PMA-treated THP-1 cells after one day of culture, while the levels of Gu/Zn-SOD and catalase were progressively decreased by Western blot analysis. In contrast, levels of Cu/Zn-SOD nd catalase protein and enzyme activity remained unchanged in THP-1 cells after transforming growth factor-β1 treatment. Cu/Zn-SOD is oxidatively inactivated by exposure to H2O2 which is produced by PMA-treated THP-1 cells, and then the inactivated enzyme undergoes proteolysis and fragmentation as analyzed by radiolabeled method. Thus monocytes have a coordinated system for synthesis and degradation of antioxidant enzymes during PMA-induced differentiation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|